Located in the foothills of the Cordillera Oriental, Private Reserve Bear Refuge is subject to local human pressure represented by deforestation, burning, grazing and hunting of wildlife. Therefore, it is necessary to know the condition of some areas through the use of organisms as bioindicators Lepidoptera (Rhopalocera). The samples included four areas of the Reserve features: montane rainforest (BPM), lower montane rain forest (BPMB), Rain Forest Premontane (BPP) and peripheral areas of the reserve, known as buffers (ZA). Biological information was analyzed under the following criteria: Representation, Diversity alpha and beta diversity. 78 individuals were collected belonging to 3 families, 12 subfamilies, 29 genera and 40 species. The Nymphalidae family presented a richer with 32 species, of which 26 belong to the subfamily Heliconiinae, 20 to the subfamily Ithomiinae, 9 Nymphalinae, 7 Satyrinae and the remainder represented by species of the subfamilies Biblidinae, Charaxinae, and Morphinae. Comparing the richness and abun-dance of species between areas, greater diversity of butterflies found in the Buffer Zone. The complementarity between the 4 areas indicates that there is a correspondence in species composition between areas. While the results showed marked differences, the knowledge we have about the diversity of the fauna of Rhopalocera (butterflies), is still incipient. So far, this study represents the first approach to a preliminary list of species in the Private Reserve Spectacled Bear Refuge.