<p>Fishing production in Colombia represents a growing economic activity. Among the most consumed species are rainbow trout (Oncorhynchus mykiss) and catfish (Brachyplatistoma sp.). The first was introduced to the country at the end of the 1930s, while the catfish is a species typical of Latin America. The objective of this research was to determine the microbiological quality of a trout and a catfish commercialized in Tunja and to make a microbiological comparison of the two samples. Selective and differential means were used to isolate pathogens such as coliforms, Salmonella spp., Vibrio spp. and Staphylococcus aureus coagulasa positive. The results showed the presence of strains of Salmonella spp., Escherichia coli, and Vibrio spp. Finally, it is concluded that the samples analyzed, despite being of different commercial origin and having completely different habitat and feeding conditions, do not meet the microbiological requirements to be marketed and could represent a potential threat to consumer health.</p>